Detecting drug effects by Fourier Transform InfraRed microspectroscopy (microFT-IR) on single cells.

Starting date
August 2, 2010
Duration (months)
6
Managers or local contacts
Bellisola Giuseppe

We will exploit some potential advantages of Fourier Transform InfraRed absorbance microspectroscoy (microFT-IR) to identify biochemical and morphological effects of drugs by SR IR microanalysis on single cells. The goal is to start developing a reference library of validated FTIR spectra via Synchrotron Radiation IR microprobe for the future application of microFT-IR analysis as a drug screening test and for clinical follow-up of patients with leukaemia. We expect to acquire the maximal number of representative spectra with acceptable signal-to-noise ratio (S/N) values for statistics significance in samples. In parallel, complementary biochemical information achieved in samples will be associated with spectral information allowing to cross-validate
reference FT-IR absorbance spectra of functional and structural cellular events induced by drugs in cancer cell models.

Project participants

Elisabetta Moratti
Claudio Sorio
Associate Professor

Collaboratori esterni

Gianfelice Cinque
Diamond Light Source - UK B22 IR beamlline Principal Beamline Sientist
Publications
Title Authors Year
Tracking InfraRed signatures of drugs in cancer cells by Fourier Transform microspectroscopy Bellisola G.; Della Peruta M.; Vezzalini M.; Moratti E.; Vaccari L.; Birarda G.;Piccinini M.; Cinque G.; Sorio C. 2010

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