Testing drug resistance/sensitivity in cancer cell lines by Fourier Transform InfraRed microspectroscopy (microFTIR) and unsupervised pattern recognition.

Starting date
May 4, 2011
Duration (months)
12
Managers or local contacts
Bellisola Giuseppe

To demonstrate that microFTIR combined with unsupervised multi-parametric analysis in spectra is a robust method to achieve rapid and accurate information on drug-sensitivity and drug-resistance in leukemic cells. The FTIR absorbance spectra of single Ba/F3 cells expressing the drug-sensitive p210BCR-ABL or the drug-resistant p210T315IBCR-ABL mutant exposed to drugs, and of blast cells isolated from patients with Chronic Myeloid Leukemia (CML) responsive and non responsive to common anti-leukemic treatment protocols will be obtained. To identify drug-sensitive and drug-resistant cells, we will apply cluster analysis (CA) to selected intervals of frequencies where signatures of interference with BCR-ABL are expected in the IR spectrum of cells exposed to different drugs. Cross-validation will be obtained by complementary analyses in samples and correlation studies will be carried out.

Project participants

Giuseppe Bellisola
Elisabetta Moratti
Claudio Sorio
Associate Professor
Marzia Vezzalini
Scholarship holder

Collaboratori esterni

Gianfelice Cinque
Diamond Light Source - UK B22 IR beamlline Principal Beamline Sientist
Sara Redaelli
Università di Milano-Bicocca Clinical Medicine and Prevention ricercatrice

Activities

Research facilities