MicroFTIR analysis of cystic fibrosis (CF) epithelial cell lines and of blood leukocytes from CF patients.

Starting date
March 15, 2012
Duration (months)
12
Managers or local contacts
Bellisola Giuseppe
Keyword
cystic fibrosis, CFTR, FTIR; synchrotron radiation, blood leukocytes

Cystic fibrosis (CF) is the most common multi-organ and life-span shorthening genetic disease involving the cystic fibrosis transmembrane conductance regulator (CFTR) protein. This anion channel regulates the secretion of components in sweat, mucus and digestive juices. Actually, some CFTR correctors/ptentiators that rescue the trafficking/functioning of CFTR mutants are in phase III clinical trials. Unfortunately, current CFTR bioassays are uncomfortable and/or scarcely practicable in CF patients.Therefore, we suggested to perform CFTR bioassays in blood leukocytes (monocytes). We intend to apply SR microFTIR spectroscopy to obtain mid-IR reference spectra representative of some human CF cell lines and of blood monocytes from CF patients in order to identify IR signatures of CFTR correctors/potentiator and to test microFTIR as a method to identify the effecacy of drugs on CFTR mutants.

Project participants

Chiara Angiari
Giuseppe Bellisola
Jan Evert Johansson
Paola Maria Melotti
Student Tutor
Elisabetta Moratti
Claudio Sorio
Associate Professor
Genny Verze'

Collaboratori esterni

Gianfelice Cinque
Diamond Light Source - UK B22 IR beamlline Principal Beamline Sientist
Giuseppe Bellisola
Azienda Ospedaliera Universitaria Integrata di Verona Patologia Dirigente medico I livello
Katia Wehbe
Diamond Light Source - UK
Paola Melotti
AOUI Verona

Activities

Research facilities