Pubblicazioni

Autori:

Nanì, S; Fumagalli, Laura; Sinha, U; Kamen, L; Scapini, Patrizia; Berton, Giorgio

Titolo:

Src Family Kinases and Syk Are Required for Neutrophil Extracellular Trap Formation in Response to β-Glucan Particles

Anno:

2015

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Referee:

Sì

Nome rivista:

JOURNAL OF INNATE IMMUNITY

ISSN Rivista:

1662-811X

N° Volume:

7

Numero o Fascicolo:

1

Intervallo pagine:

59-73

Parole chiave:

β-glucan; neutrophil extracellular trap (NET); Src family kinases

Breve descrizione dei contenuti:

We report that particles of β-glucan, one of the surface components of yeasts, are powerful inducers of neutrophil extracellular trap (NET) formation in human neutrophils. β-Glucan triggered a prolonged phosphorylation of Src family kinases and Syk that were suppressed by the Src family inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3, 4-d] pyrimidine (PP2) and a novel Syk inhibitor, PRT-060318, respectively. PP2 and PRT-060318 also inhibited β-glucan-induced NET formation and reactive oxygen species (ROS) generation, suggesting that both responses are triggered by a Src/Syk-regulated signaling pathway. Given that the NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI) markedly inhibited NET formation, our findings suggest that ROS are required for the full-blown formation of NETs in response to β-glucan particles. Contrary to β-glucan, ROS generation triggered by phorbol myristate acetate (PMA) was unaffected by PP2 and PRT-060318, but these compounds, as well as DPI, suppressed Src/Syk phosphorylation triggered by PMA. Whereas PP2 had no effect on PMA-induced NET formation, PRT-060318 had a significant, albeit partial, inhibitory effect, thus suggesting that ROS induce NET formation in part via activation of Syk. These findings were substantiated by the evidence that neutrophils from mice with the conditional deletion of Syk were defective in formation of NETs in response to β-glucan

Id prodotto:

83189

Handle IRIS:

11562/799565

depositato il:

9 gennaio 2015

ultima modifica:

16 novembre 2022

Citazione bibliografica:

Nanì, S; Fumagalli, Laura; Sinha, U; Kamen, L; Scapini, Patrizia; Berton, Giorgio, Src Family Kinases and Syk Are Required for Neutrophil Extracellular Trap Formation in Response to β-Glucan Particles «JOURNAL OF INNATE IMMUNITY» , vol. 7 , n. 1 , 2015 , pp. 59-73

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