Detecting drug effects by Fourier Transform InfraRed microspectroscopy (microFT-IR) on single cells.

Starting date
August 2, 2010
Duration (months)
Department of Engineering for Innovation Medicine
Managers or local contacts
Bellisola Giuseppe

We will exploit some potential advantages of Fourier Transform InfraRed absorbance microspectroscoy (microFT-IR) to identify biochemical and morphological effects of drugs by SR IR microanalysis on single cells. The goal is to start developing a reference library of validated FTIR spectra via Synchrotron Radiation IR microprobe for the future application of microFT-IR analysis as a drug screening test and for clinical follow-up of patients with leukaemia. We expect to acquire the maximal number of representative spectra with acceptable signal-to-noise ratio (S/N) values for statistics significance in samples. In parallel, complementary biochemical information achieved in samples will be associated with spectral information allowing to cross-validate
reference FT-IR absorbance spectra of functional and structural cellular events induced by drugs in cancer cell models.

Project participants

Elisabetta Moratti
Claudio Sorio
Associate Professor

Collaboratori esterni

Gianfelice Cinque
Diamond Light Source - UK B22 IR beamlline Principal Beamline Sientist
Title Authors Year
Tracking InfraRed signatures of drugs in cancer cells by Fourier Transform microspectroscopy Bellisola, Giuseppe; DELLA PERUTA, Marco; Vezzalini, Marzia; Moratti, Elisabetta; Vaccari, L.; Birarda, G.; Piccinini, M.; Cinque, G.; Sorio, Claudio 2010




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