Testing drug resistance/sensitivity in cancer cell lines by Fourier Transform InfraRed microspectroscopy (microFTIR) and unsupervised pattern recognition.
- Testing drug resistance/sensitivity in cancer cell lines by Fourier Transform InfraRed microspectroscopy (microFTIR) and unsupervised pattern recognition.
- Starting date
- May 4, 2011
- Duration (months)
- Managers or local contacts
To demonstrate that microFTIR combined with unsupervised multi-parametric analysis in spectra is a robust method to achieve rapid and accurate information on drug-sensitivity and drug-resistance in leukemic cells. The FTIR absorbance spectra of single Ba/F3 cells expressing the drug-sensitive p210BCR-ABL or the drug-resistant p210T315IBCR-ABL mutant exposed to drugs, and of blast cells isolated from patients with Chronic Myeloid Leukemia (CML) responsive and non responsive to common anti-leukemic treatment protocols will be obtained. To identify drug-sensitive and drug-resistant cells, we will apply cluster analysis (CA) to selected intervals of frequencies where signatures of interference with BCR-ABL are expected in the IR spectrum of cells exposed to different drugs. Cross-validation will be obtained by complementary analyses in samples and correlation studies will be carried out.
Diamond Light Source - UK
B22 IR beamlline
Principal Beamline Sientist
Università di Milano-Bicocca
Clinical Medicine and Prevention
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