Pubblicazioni

Autori:

Vezzalini, Marzia; Aletta, John M; Beghelli, Stefania; Moratti, Elisabetta; Della Peruta, Marco; Mafficini, Andrea; Mojica, Wilfrido D; Mombello, Aldo; Scarpa, Aldo; Sorio, Claudio

Titolo:

In situ analysis of methylarginine

Anno:

2010

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

A Stampa

Referee:

Sì

Nome rivista:

Histopathology

ISSN Rivista:

0309-0167

N° Volume:

57

Numero o Fascicolo:

5

Intervallo pagine:

725-733

Parole chiave:

cancer, methylarginine, normal tissue, PRMT, immunohistochemistry

Breve descrizione dei contenuti:

Aims: To (i) determine whether methylarginine-specific antibodies can be employed for standard immuno- histochemical analysis of paraffin-embedded tissues, (ii) analyse methylarginine expression in normal and neoplastic tissues and (iii) correlate methylarginine expression with that of protein arginine methyltrans- ferase (PRMT1), the predominant cellular arginine methyltransferase. Methods and results: Immunohistochemistry of normal and cancer tissues was performed utilizing three commercial polyclonal antibodies: anti-methylargi- nine-specific antibody (anti-mRG) raised against a methylarginine peptide, Control antibody (anti-RG), a control antiserum raised against a corresponding arginine peptide without any methylated residues and anti-PRMT1. Nuclear and ⁄ or cytoplasmic methylargi- nine expression was detected in all keratinized and non-keratinized epithelia. A preliminary survey of a series of thyroid, pancreatic, colonic and gastric can- cers identified a different pattern of methylarginine expression in comparison with normal tissue. A corre- lation between methylarginine staining and PRMT1 expression was found in all normal and cancer tissues analysed. Conclusion: Methylarginine-specific antibodies are capable of recognizing methylarginine proteins (MeRP) in paraffin-embedded tissues. Methylarginine proteins are expressed widely and show differences in subcellular localization in various organs and neo- plastic conditions. The efficient detection of methyl- proteins by standard immunohistochemistry provides a new tool to investigate the role of methylarginine proteins (MeRP) in biological processes including carcinogenesis.

Id prodotto:

119883

Handle IRIS:

11562/1038493

ultima modifica:

15 novembre 2022

Citazione bibliografica:

Vezzalini, Marzia; Aletta, John M; Beghelli, Stefania; Moratti, Elisabetta; Della Peruta, Marco; Mafficini, Andrea; Mojica, Wilfrido D; Mombello, Aldo; Scarpa, Aldo; Sorio, Claudio, In situ analysis of methylarginine «Histopathology» , vol. 57 , n. 5 , 2010 , pp. 725-733

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