Publications

Authors:

Ruggiero, Alessandra; Piubelli, Chiara; Calciano, Lucia; Accordini, Simone; Valenti, Maria Teresa; Dalle Carbonare, Luca; Siracusano, Gabriel; Temperton, Nigel; Tiberti, Natalia; Longoni, Silvia Stefania; Pizzato, Massimo; Accordini, Silvia; A. M. S. L. V., Group; Fantoni, Tobia; Lopalco, Lucia; Beretta, Alberto; Bisoffi, Zeno; Zipeto, Donato

Title:

SARS-CoV-2 vaccination elicits unconventional IgM specific responses in naïve and previously COVID-19-infected individuals

Year:

2022

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

Sì

Name of journal:

EBIOMEDICINE

ISSN of journal:

2352-3964

N° Volume:

77

Page numbers:

1-13

Keyword:

BTN162b2 vaccine, COVID-19, IgG, IgM, SARS-CoV-2, Spike

Short description of contents:

Background: Currently, evaluation of the IgG antibodies specific for the SARS-CoV-2 Spike protein following vaccination is used worldwide to estimate vaccine response. Limited data are available on vaccine-elicited IgM antibodies and their potential implication in immunity to SARS-CoV-2. Methods: We performed a longitudinal study to quantify anti-S SARS-CoV-2 IgG and IgM (IgG-S and IgM-S) in health care worker (HCW) recipients of the BNT162b2 vaccine. Samples were collected before administration (T0), at the second dose (T1) and three weeks after T1 (T2). The cohort included 1584 immunologically naïve to SARS-CoV-2 (IN) and 289 with history of previous infection (PI). Findings: IN showed three patterns of responses: (a) IgG positive/IgM negative (36.1%), (b) coordinated IgM-S/IgG-S responses appearing at T1 (37.4%) and (c) IgM appearing after IgG (26.3%). Coordinated IgM-S/IgG-S responses were associated with higher IgG titres. In IgM-S positive PI, 64.5% were IgM-S positive before vaccination, whereas 32% and 3.5% developed IgM-S after the first and second vaccine dose, respectively. IgM-S positive sera had higher pseudovirus neutralization titres compared to the IgM-S negative. Interpretation: Coordinated expression of IgG-S and IgM-S after vaccination was associated with a significantly more efficient response in both antibody levels and virus-neutralizing activity. The unconventional IgG-S positive/IgM-S negative responses may suggest a recruitment of cross coronaviruses immunity by vaccination, warranting further investigation. Funding: Italian Ministry of Health under "Fondi Ricerca Corrente"- L1P5 and "Progetto Ricerca Finalizzata COVID-2020-12371675"; FUR 2020 Department of Excellence 2018-2022, MIUR, Italy; The Brain Research Foundation Verona.

Note:

Zeno Bisoffi and Donato Zipeto contributed equally to this work.

Web page:

https://doi.org/10.1016/j.ebiom.2022.103888

Product ID:

125063

Handle IRIS:

11562/1058496

Last Modified:

January 25, 2025

Bibliographic citation:

Ruggiero, Alessandra; Piubelli, Chiara; Calciano, Lucia; Accordini, Simone; Valenti, Maria Teresa; Dalle Carbonare, Luca; Siracusano, Gabriel; Temperton, Nigel; Tiberti, Natalia; Longoni, Silvia Stefania; Pizzato, Massimo; Accordini, Silvia; A. M. S. L. V., Group; Fantoni, Tobia; Lopalco, Lucia; Beretta, Alberto; Bisoffi, Zeno; Zipeto, Donato, SARS-CoV-2 vaccination elicits unconventional IgM specific responses in naïve and previously COVID-19-infected individuals «EBIOMEDICINE» , vol. 77 , 2022 , pp. 1-13

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo