Publications

Authors:

Toffali, Lara; D'Ulivo, Beatrice; Giagulli, Cinzia; Montresor, Alessio; Zenaro, Elena; Delledonne, Massimo; Rossato, Marzia; Iadarola, Barbara; Sbarbati, Andrea; Bernardi, Paolo; Angelini, Gabriele; Rossi, Barbara; Lopez, Nicola; Linke, Wolfgang A; Unger, Andreas; Di Silvestre, Dario; Benazzi, Louise; De Palma, Antonella; Motta, Sara; Constantin, Gabriela; Mauri, Pierluigi; Laudanna, Carlo

Title:

An isoform of the giant protein titin is a master regulator of human T lymphocyte trafficking

Year:

2023

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

Sì

Name of journal:

CELL REPORTS

ISSN of journal:

2211-1247

N° Volume:

42

Number or Folder:

5

Page numbers:

1-29

Keyword:

CP: Immunology; adhesion; cell deformability; chemokine; integrin activation; leukocyte trafficking; microvilli; migration; selectin; signal transduction; titin

Short description of contents:

Response to multiple microenvironmental cues and resilience to mechanical stress are essential features of trafficking leukocytes. Here, we describe unexpected role of titin (TTN), the largest protein encoded by the human genome, in the regulation of mechanisms of lymphocyte trafficking. Human T and B lymphocytes express five TTN isoforms, exhibiting cell-specific expression, distinct localization to plasma membrane microdomains, and different distribution to cytosolic versus nuclear compartments. In T lymphocytes, the LTTN1 isoform governs the morphogenesis of plasma membrane microvilli independently of ERM protein phosphorylation status, thus allowing selectin-mediated capturing and rolling adhesions. Likewise, LTTN1 controls chemokine-triggered integrin activation. Accordingly, LTTN1 mediates rho and rap small GTPases activation, but not actin polymerization. In contrast, chemotaxis is facilitated by LTTN1 degradation. Finally, LTTN1 controls resilience to passive cell deformation and ensures T lymphocyte survival in the blood stream. LTTN1 is, thus, a critical and versatile housekeeping regulator of T lymphocyte trafficking.

Web page:

https://doi.org/10.1016/j.celrep.2023.112516

Product ID:

133858

Handle IRIS:

11562/1094287

Last Modified:

January 27, 2025

Bibliographic citation:

Toffali, Lara; D'Ulivo, Beatrice; Giagulli, Cinzia; Montresor, Alessio; Zenaro, Elena; Delledonne, Massimo; Rossato, Marzia; Iadarola, Barbara; Sbarbati, Andrea; Bernardi, Paolo; Angelini, Gabriele; Rossi, Barbara; Lopez, Nicola; Linke, Wolfgang A; Unger, Andreas; Di Silvestre, Dario; Benazzi, Louise; De Palma, Antonella; Motta, Sara; Constantin, Gabriela; Mauri, Pierluigi; Laudanna, Carlo, An isoform of the giant protein titin is a master regulator of human T lymphocyte trafficking «CELL REPORTS» , vol. 42 , n. 5 , 2023 , pp. 1-29

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