Pubblicazioni

Autori:

Gobbo, Stefano; Eccher, Albino; Gallina, Sebastian; D'Aietti, Damiano; Princiotta, Alessandro; Ditonno, Francesco; Tafuri, Alessandro; Cerruto, Maria A; Marletta, Stefano; Sanguedolce, Francesca; Scarpa, Aldo; Brunelli, Matteo; Antonelli, Alessandro

Titolo:

Validation of real-time prostatic biopsies evaluation with fluorescence laser confocal microscopy

Anno:

2023

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Referee:

No

Nome rivista:

MINERVA UROLOGY AND NEPHROLOGY

ISSN Rivista:

2724-6442

N° Volume:

75

Numero o Fascicolo:

5

Intervallo pagine:

577-582

Parole chiave:

Prostatic neoplasms; Microscopy, fluorescence, multiphoton; Pathology; Diagnostic techniques, urological

Breve descrizione dei contenuti:

Background: Routine processing of prostate biopsies requires conventional steps that usually take a few days. The aim of this study was to validate the use of fluorescence laser confocal microscopy (FCM) for real-time diagnostics. Methods: We prospectively tested images from prostate needle biopsies (75 images were evaluated by FCM and conventional slides). Two pathologists reviewed the images and assessed agreements between FCM versus conventional slides and between pathologists (κ-values). Interpretation was performed on digital images from the VivaScope 2500 confocal microscope (MAVIG GmbH, Munich, Germany; Caliber I.D., Rochester, NY, USA) placed in the urological operating room. Cancerous versus benign tissue was the primary focus, then the application of the grading system. Results: Cancer was diagnosed in 24 conventional slides (on 75 images) in which agreement among pathologists was high for both conventional (κ=0.96) and FMC (κ=0.84). 1/24 (4%) was ISUP/WHO grade group I, 12/24 (50%) II, 8/24 (33%) III, 2/24 (8%) IV and 1/24 (4%) grade V. Near perfect agreement was obtained for grades I, IV and V (κ=0.85). Grade III values achieved a moderate agreement (κ=0.55). The mean time for laser scanning was 9 minutes. For the remaining non-tumor images, agreement was nearly perfect (κ=0.81). Conclusions: We validated the use of FCM for real-time cancer detection in prostate biopsies.

Id prodotto:

134795

Handle IRIS:

11562/1100446

ultima modifica:

29 settembre 2024

Citazione bibliografica:

Gobbo, Stefano; Eccher, Albino; Gallina, Sebastian; D'Aietti, Damiano; Princiotta, Alessandro; Ditonno, Francesco; Tafuri, Alessandro; Cerruto, Maria A; Marletta, Stefano; Sanguedolce, Francesca; Scarpa, Aldo; Brunelli, Matteo; Antonelli, Alessandro, Validation of real-time prostatic biopsies evaluation with fluorescence laser confocal microscopy «MINERVA UROLOGY AND NEPHROLOGY» , vol. 75 , n. 5 , 2023 , pp. 577-582

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