Publications

Authors:

Pettinella, Francesca; Mariotti, Barbara; Lattanzi, Chiara; Bruderek, Kirsten; Donini, Marta; Costa, Sara; Marini, Olivia; Iannoto, Giulia; Gasperini, Sara; Caveggion, Elena; Castellucci, Monica; Calzetti, Federica; Bianchetto-Aguilera, Francisco; Gardiman, Elisa; Giani, Matteo; Dusi, Stefano; Cantini, Maurizio; Vassanelli, Aurora; Pavone, Denise; Milella, Michele; Pilotto, Sara; Biondani, Pamela; Höing, Benedikt; Schleupner, Marie Carolin; Hussain, Timon; Hadaschik, Boris; Kaspar, Cordelia; Visco, Carlo; Tecchio, Cristina; Koenderman, Leo; Bazzoni, Flavia; Tamassia, Nicola; Brandau, Sven; Cassatella, Marco A.; Scapini, Patrizia

Title:

Surface CD52, CD84, and PTGER2 mark mature PMN-MDSCs from cancer patients and G-CSF-treated donors

Year:

2024

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

No

Name of journal:

CELL REPORTS MEDICINE

ISSN of journal:

2666-3791

N° Volume:

5

Number or Folder:

2

Page numbers:

1-28

Keyword:

G-CSF; PMN-MDSCs; biomarkers; cancer patients; neutrophils

Short description of contents:

: Precise molecular characterization of circulating polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) is hampered by their mixed composition of mature and immature cells and lack of specific markers. Here, we focus on mature CD66b+CD10+CD16+CD11b+ PMN-MDSCs (mPMN-MDSCs) from either cancer patients or healthy donors receiving G-CSF for stem cell mobilization (GDs). By RNA sequencing (RNA-seq) experiments, we report the identification of a distinct gene signature shared by the different mPMN-MDSC populations under investigation, also validated in mPMN-MDSCs from GDs and tumor-associated neutrophils (TANs) by single-cell RNA-seq (scRNA-seq) experiments. Analysis of such a gene signature uncovers a specific transcriptional program associated with mPMN-MDSC differentiation and allows us to identify that, in patients with either solid or hematologic tumors and in GDs, CD52, CD84, and prostaglandin E receptor 2 (PTGER2) represent potential mPMN-MDSC-associated markers. Altogether, our findings indicate that mature PMN-MDSCs distinctively undergo specific reprogramming during differentiation and lay the groundwork for selective immunomonitoring, and eventually targeting, of mature PMN-MDSCs.

Product ID:

137801

Handle IRIS:

11562/1119514

Last Modified:

October 16, 2024

Bibliographic citation:

Pettinella, Francesca; Mariotti, Barbara; Lattanzi, Chiara; Bruderek, Kirsten; Donini, Marta; Costa, Sara; Marini, Olivia; Iannoto, Giulia; Gasperini, Sara; Caveggion, Elena; Castellucci, Monica; Calzetti, Federica; Bianchetto-Aguilera, Francisco; Gardiman, Elisa; Giani, Matteo; Dusi, Stefano; Cantini, Maurizio; Vassanelli, Aurora; Pavone, Denise; Milella, Michele; Pilotto, Sara; Biondani, Pamela; Höing, Benedikt; Schleupner, Marie Carolin; Hussain, Timon; Hadaschik, Boris; Kaspar, Cordelia; Visco, Carlo; Tecchio, Cristina; Koenderman, Leo; Bazzoni, Flavia; Tamassia, Nicola; Brandau, Sven; Cassatella, Marco A.; Scapini, Patrizia, Surface CD52, CD84, and PTGER2 mark mature PMN-MDSCs from cancer patients and G-CSF-treated donors «CELL REPORTS MEDICINE» , vol. 5 , n. 2 , 2024 , pp. 1-28

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