Publications

Authors:

Biasi, Domenico; Bambara, Lisa Maria; Carletto, Antonio; Caraffi, M; Serra, Maria Cristina; Chirumbolo, Salvatore; Bellavite, Paolo

Title:

Factor-specific changes in oxidative burst response of human neutrophils in skin-window exudates

Year:

1993

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

Inflammation

ISSN of journal:

0360-3997

N° Volume:

17

Number or Folder:

1

Page numbers:

13-23

Keyword:

Neutrophil; receptors; chemotaxis; inflammation

Short description of contents:

Human neutrophils were isolated from blood and aseptic inflammatory exudates. The respiratory burst response was measured as superoxide (O2-) production by a microplate assay system and polarographically as oxygen consumption. Exudate cells exhibited a respiratory burst in response to n-formyl-methionyl-leucyl-phenyl-alanine (FMLP) that was two- to threefold higher than the burst exhibited by peripheral blood cells. The O2- production induced by substance P was also found to be fivefold higher in exudate cells, while the metabolic response to other stimulants such as concanavalin A (con A), phorbol-myristate acetate (PMA), NaF, and immunocomplexes was not primed. Serum-treated zymosan (STZ)-stimulated activity was primed by only 11%. In contrast, superoxide production in response to tumor necrosis factor-alpha (TNF) was decreased in exudate versus blood cells by about 50%. Therefore, the skin-window cells, compared to blood cells, appear to be at the same time primed, unmodified, and desensitized, according to the different stimulants employed.

Product ID:

1146

Handle IRIS:

11562/1146

Deposited On:

May 5, 2010

Last Modified:

November 15, 2022

Bibliographic citation:

Biasi, Domenico; Bambara, Lisa Maria; Carletto, Antonio; Caraffi, M; Serra, Maria Cristina; Chirumbolo, Salvatore; Bellavite, Paolo, Factor-specific changes in oxidative burst response of human neutrophils in skin-window exudates «Inflammation» , vol. 17 , n. 1 , 1993 , pp. 13-23

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