Publications

Authors:

D. Girelli; O. Olivieri; A. M. Stanzial; P. Guarini; M. T. Trevisan; A. Bassi; R. Corrocher

Title:

Factors affecting the thiobarbituric acid test as index of red blood cell susceptibility to lipid peroxidation: a multivariate analysis

Year:

1994

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Nations of authors:

ITALIA

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

Clinica Chimica Acta

ISSN of journal:

0009-8981

N° Volume:

227

Number or Folder:

1-2

Page numbers:

45-57

Short description of contents:

The relationship between formation of thiobarbituric acid reactive substances (TBARS) in red blood cells (RBC) after exposure to H2O2 and factors potentially able to modulate it was investigated by a multivariate analysis in 92 healthy volunteers. The independent covariates considered were: RBC membrane fatty acids and cholesterol, RBC antioxidant enzymes and zinc, plasma vitamins A and E and serum selenium, zinc and copper. The stepwise multiple-linear-regression analysis revealed RBC membrane fatty acids and cholesterol as predictors of a consistent proportion of the RBC-TBARS variability whereas none of the antioxidants entered the equation. The unsaturation index was the most important individual predictor; RBC-TBARS increased with increasing concentrations of total omega-3 polyunsaturated fatty acids, C 20:5 omega-3 and cholesterol, whereas they decreased with increasing concentrations of total monounsaturated fatty acids, saturated fatty acids, C 16:0 and C 18:0. It is suggested that formation of TBARS, at least in currently used conditions, reflects mainly the lipid composition of the tissue under investigation, without giving sufficient information about the status of the antioxidant defences.

Product ID:

2372

Handle IRIS:

11562/2372

Deposited On:

July 20, 2007

Last Modified:

November 2, 2016

Bibliographic citation:

D. Girelli; O. Olivieri; A. M. Stanzial; P. Guarini; M. T. Trevisan; A. Bassi; R. Corrocher, Factors affecting the thiobarbituric acid test as index of red blood cell susceptibility to lipid peroxidation: a multivariate analysis «Clinica Chimica Acta» , vol. 227 , n. 1-2 , 1994 , pp. 45-57

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