Publications

Authors:

D. Girelli; A. Lupo; M. T. Trevisan; O. Olivieri; P. Bernich; P. Zorzan; A. Bassi; A. M. Stanzial; S. Ferrari; R. Corrocher

Title:

Red blood cell susceptibility to lipid peroxidation, membrane lipid composition, and antioxidant enzymes in continuous ambulatory peritoneal dialysis patients

Year:

1992

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Nations of authors:

ITALIA

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

Peritoneal Dialysis International

ISSN of journal:

0896-8608

N° Volume:

12

Page numbers:

196-205

Short description of contents:

OBJECTIVE: To investigate the overall susceptibility of red blood cells (RBC) to lipid peroxidation from patients on continuous ambulatory peritoneal dialysis (CAPD). METHODS: The following parameters were measured: RBC malondialdehyde (MDA) production after oxidative stress with H2O2, RBC antioxidant enzymes glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), and RBC membrane lipid composition. The levels of plasma vitamin E and serum selenium were also assayed. PATIENTS: Eleven patients on continuous ambulatory peritoneal dialysis. Twenty-one healthy blood donors of similar age were used as normal controls. RESULTS: The MDA formation after H2O2 stimulation was normal in CAPD patients (0.79 +/- 0.1 mumol/gHb versus 0.78 +/- 0.1 in the control group). RBC from CAPD patients also showed a normal SOD activity, a more than adequate vitamin E status, and a peculiar pattern of membrane lipids, with reduced polyunsaturated fatty acids (p less than 0.001) and increased monounsaturated fatty acids (p less than 0.001). Both RBC GSH-Px activity, a selenium-dependent enzyme, and serum selenium levels were significantly lower in CAPD patients, and a significant positive correlation (r = 0.68; p less than 0.02) between the two parameters was found. CONCLUSIONS: This study found a normal sensitivity to oxidant stress in RBC from a group of CAPD patients, despite an impaired GSH-Px activity. The peculiar lipid pattern of RBC membrane, characterized by reduced PUFA and increased MUFA content, may contribute, in addition to adequate SOD activity and vitamin E status, to normal RBC lipid peroxidation.

Product ID:

2383

Handle IRIS:

11562/2383

Deposited On:

August 8, 2007

Last Modified:

November 2, 2016

Bibliographic citation:

D. Girelli; A. Lupo; M. T. Trevisan; O. Olivieri; P. Bernich; P. Zorzan; A. Bassi; A. M. Stanzial; S. Ferrari; R. Corrocher, Red blood cell susceptibility to lipid peroxidation, membrane lipid composition, and antioxidant enzymes in continuous ambulatory peritoneal dialysis patients «Peritoneal Dialysis International» , vol. 12 , 1992 , pp. 196-205

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