Publications

Authors:

Copreni, E.; Nicolis, Elena; Tamanini, Anna; Bezzerri, Valentino; Castellani, S.; Palmieri, L.; Giri, Mariagrazia; Vella, Antonio; Colombatti, Marco; Rizzotti, P.; Conese, M.; Cabrini, Giulio

Title:

Late generation lentiviral vectors: evaluation of inflammatory potential in human airway epithelial cells.

Year:

2009

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

No

Name of journal:

VIRUS RESEARCH

ISSN of journal:

0168-1702

N° Volume:

144

Number or Folder:

1-2

Page numbers:

8-17

Keyword:

nf-kappa-b; mediated gene-transfer; c chemokine ip-10; cystic-fibrosis; in-vivo; immune-responses; respiratory cells; adenoviral vectors; icam-1 induction; expression

Short description of contents:

Lentiviruses (LVs) are considered one of the most promising tools for gene transfer, however, their potential to induce pro-inflammatory cytokines on delivery into the respiratory tissue remains to be established. Here we tested a third-generation vesicular stomatitis virus (VSV)-G pseudotyped LV vector in the two respiratory epithelial cell lines A549 and CFT1-C2. We observed that the VSV-G LV vector does not induce (a) activation of the nuclear factor (NF)-kappaB, which intervenes in transcription of pro-inflammatory genes; (b) expression of ICAM-1; and (c) transcription of a panel of cytokines, with the exception of a mild and transient (24h) increase of IFN-gamma mRNA. In contrast, an adenovirus-derived vector strongly activated NF-kappaB and different transcripts such as those of ICAM-1, IL-8, RANTES, IP-10, TNF-alpha, IL-6, IL-1 beta. In conclusion, this third-generation VSV-G pseudotyped LV vector does not elicit major pro-inflammatory signals in human airway epithelial cells and appears to be better suited for gene delivery strategies.

Product ID:

53626

Handle IRIS:

11562/334944

Deposited On:

September 8, 2014

Last Modified:

November 15, 2022

Bibliographic citation:

Copreni, E.; Nicolis, Elena; Tamanini, Anna; Bezzerri, Valentino; Castellani, S.; Palmieri, L.; Giri, Mariagrazia; Vella, Antonio; Colombatti, Marco; Rizzotti, P.; Conese, M.; Cabrini, Giulio, Late generation lentiviral vectors: evaluation of inflammatory potential in human airway epithelial cells. «VIRUS RESEARCH» , vol. 144 , n. 1-2 , 2009 , pp. 8-17

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