Publications

Microarray analysis on human neuroblastoma cells exposed to aluminum, ²(1-42)-amyloid or the ²(1-42)-amyloid aluminum complex.  (2011)

Authors:
Gatta V; Drago D; Fincati K; Valenti MT; Dalle Carbonare L; Sensi SL; Zatta P.
Title:
Microarray analysis on human neuroblastoma cells exposed to aluminum, ²(1-42)-amyloid or the ²(1-42)-amyloid aluminum complex.
Year:
2011
Type of item:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Nations of authors:
ITALIA
Language:
Inglese
Format:
Elettronico
Referee:
Name of journal:
PLoS ONE
ISSN of journal:
1932-6203
N° Volume:
6
Number or Folder:
1
Page numbers:
1-16
Code PMID:
21298039
Keyword:
amyloid, neuroblastoma, microarray
Short description of contents:
Background: A typical pathological feature of Alzheimer's disease (AD) is the appearance in the brain of senile plaques made up of b-amyloid (Ab) and neurofibrillary tangles. AD is also associated with an abnormal accumulation of some metal ions, and we have recently shown that one of these, aluminum (Al), plays a relevant role in affecting Ab aggregation and neurotoxicity. Methodology: In this study, employing a microarray analysis of 35,129 genes, we investigated the effects induced by the exposure to the Ab142-Al (Ab-Al) complex on the gene expression profile of the neuronal-like cell line, SH-SY5Y. Principal Findings: The microarray assay indicated that, compared to Ab or Al alone, exposure to Ab-Al complex produced selective changes in gene expression. Some of the genes selectively over or underexpressed are directly related to AD. A further evaluation performed with Ingenuity Pathway analysis revealed that these genes are nodes of networks and pathways that are involved in the modulation of Ca2+ homeostasis as well as in the regulation of glutamatergic transmission and synaptic plasticity. Conclusions and Significance: Ab-Al appears to be largely involved in the molecular machinery that regulates neuronal as well as synaptic dysfunction and loss. Ab-Al seems critical in modulating key AD-related pathways such as glutamatergic transmission, Ca2+ homeostasis, oxidative stress, inflammation, and neuronal apoptosis.
Product ID:
61871
Handle IRIS:
11562/362911
Deposited On:
February 21, 2016
Last Modified:
November 2, 2016
Attachments:
1
Bibliographic citation:
Gatta V; Drago D; Fincati K; Valenti MT; Dalle Carbonare L; Sensi SL; Zatta P., Microarray analysis on human neuroblastoma cells exposed to aluminum, ²(1-42)-amyloid or the ²(1-42)-amyloid aluminum complex. «PLoS ONE» , vol. 6 , n. 12011pp. 1-16

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STUDIO DI ESPRESSIONE GENICA NELLA PATOLOGIA NEURODEGENERATIVA DI ALZHEIMER Department Medicina Luca Giuseppe Dalle Carbonare
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