Publications

Authors:

Vermi, William; Micheletti, Alessandra; Finotti, Giulia; Tecchio, Cristina; Calzetti, Federica; Costa, Sara; Bugatti, Mattia; Calza, Stefano; Agostinelli, Claudio; Pileri, Stefano; Balzarini, Piera; Tucci, Alessandra; Rossi, Giuseppe; Furlani, Lara; Todeschini, Giuseppe; Zamò, Alberto; Facchetti, Fabio; Lorenzi, Luisa; Lonardi, Silvia; Cassatella, Marco A

Title:

slan+ monocytes and macrophages mediate CD20-dependent B cell lymphoma elimination via ADCC and ADCP

Year:

2018

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

No

Name of journal:

Cancer Research

ISSN of journal:

0008-5472

N° Volume:

78

Number or Folder:

13

Page numbers:

3544-3559

Keyword:

lan+ -monocytes, DLBCL, macrophages, ADCC, ADCP

Short description of contents:

Terminal tissue differentiation and function of slan+ monocytes in cancer is largely unexplored. Our recent studies demonstrated that slan+ monocytes differentiate into a distinct subset of dendritic cells (DC) in human tonsils and that slan+ cells colonize metastatic carcinoma-draining lymph nodes. Herein, we report by retrospective analysis of multi-institutional cohorts that slan+ cells infiltrate various types of non-Hodgkin lymphomas (NHL), particularly the diffuse large B-cell lymphoma (DLBCL) group, including the most aggressive, nodal and extra-nodal, forms. Nodal slan+ cells displayed features of either immature DC or macrophages, in the latter case ingesting tumor cells and apoptotic bodies. We also found in DLBCL patients that peripheral blood slan+ monocytes, but not CD14+ monocytes, increased in number and displayed highly efficient Rituximab (RTX)-mediated antibody dependent cellular cytotoxicity (ADCC), almost equivalent to that exerted by NK cells. Notably, slan+ monocytes cultured in conditioned medium from nodal DLBCL (DCM) acquired a macrophage-like phenotype, retained CD16 expression and became very efficient in RTX-mediated antibody-dependent cellular phagocytosis (ADCP). Macrophages derived from DCM-treated CD14+ monocytes performed very efficient RTX-mediated ADCP, however using different FcγRs from those used by slan+ macrophages. Our observations shed new light on the complexity of the immune microenvironment of DLBCL and demonstrate plasticity of slan+ monocytes homing to cancer tissues. Altogether, data identify slan+ monocytes and macrophages as prominent effectors of antibody-mediated tumor cell targeting in DLBCL patients.

Note:

pii: canres.2344.2017

Product ID:

102806

Handle IRIS:

11562/979775

Last Modified:

November 14, 2022

Bibliographic citation:

Vermi, William; Micheletti, Alessandra; Finotti, Giulia; Tecchio, Cristina; Calzetti, Federica; Costa, Sara; Bugatti, Mattia; Calza, Stefano; Agostinelli, Claudio; Pileri, Stefano; Balzarini, Piera; Tucci, Alessandra; Rossi, Giuseppe; Furlani, Lara; Todeschini, Giuseppe; Zamò, Alberto; Facchetti, Fabio; Lorenzi, Luisa; Lonardi, Silvia; Cassatella, Marco A, slan+ monocytes and macrophages mediate CD20-dependent B cell lymphoma elimination via ADCC and ADCP «Cancer Research» , vol. 78 , n. 13 , 2018 , pp. 3544-3559

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