Speaker:
Dr. Alessandro Martino
- Bio- Rad Laboratories
Monday, December 5, 2016
at
2:00 PM
Aula E Lente Didattica
Digital PCR is a breakthrough technology that provides ultrasensitive and absolute nucleic acid quantification. It is particularly useful for low abundance targets, targets in complex backgrounds, allelic variants (SNPs), and for monitoring subtle changes in target levels that cannot be detected with traditional quantitative PCR (qPCR). Bio-Rad QX200TM Droplet Digital PCR (ddPCR) System employs advanced droplet technology to partition a DNA sample mix in 20,000 droplets. PCR amplification is based on classical qPCR chemistries like hydrolysis probes (TaqMan) and/or intercalating dyes such as EvaGreen and occurs in each individual droplet where the target is present. Counting the positive droplets gives a very precis and absolute target quantification. By leading the digital PCR market, the Bio-Rad QX200TM ddPCR PCR System has found applications in many different fields as attested by over 400 indexed publications in the lasts years. This seminar will illustrate the basis of the ddPCR technology and its most important applications such as the analysis of somatic mutations, the quantification of copy number variations, the analysis of gene expression down to single cell level, miRNAs analysis and the quantification of genome editing events.
Dr. Alessandro Martino, Ph.D.
ddPCR Sales &
Support Specialist
Southern Europe
Bio- Rad Laboratories
The seminar will take place in the
Aula E Lente Didattica Policlinico B.go Roma
Piazzale L. Scuro, 10 - 37134 Verona
Local organization and contact:
Dr.ssa Maria Teresa Valenti
mariateresa.valenti@univr.it
Prof. Massimo Delledonne
massimo.delledonne@univr.it
- Programme Director
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External reference
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- Publication date
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November 24, 2016
pdf (it, 345 KB, 28/11/16)
