Detecting signatures of drugs in the spectra of individual leukemic cells analyzed with combined FTIR and Raman spectroscopies.

Data inizio
6 luglio 2012
Durata (mesi) 
8
Responsabili (o referenti locali)
Bellisola Giuseppe
URL
http://sunset.synchrotron-soleil.fr/sun/
Parole chiave
synchrotron radiation, infrared microspectroscopy, raman microspectroscopy, chronic myeloid leukemia, tyrosine kinase inhibitors, receptor-like protein tyrosine phosphatase gamma, drug-sensitivity, drug-resistance

Chronic Myeloid Leukemia (CML) cell models expressing drug-sensitive and drug-resistant forms of Bcr-Abl exposed to the action of tyrosine kinase inhibitors are analyzed by SR FTIR and Raman microspectroscopies to study the effects of drugs targeting abnormal Bcr-Abl associated tyrosine kinase activity characterizing those leukemic cells. The regions of phosphates (1290-900 cm-1) and amides (1700-1480 cm-1) ara analyzed and compared in the SR FTIR absorbance spectra of individual cells in order to identify IR changes related to the actions of drugs. The results of complementary investigations carried out in parallel samples will cross-validate the results of IR analysis. We expect to obtain cross-validated FTIR and Raman reference spectra of drug-sensitive and drug resistant CML cell models.

Partecipanti al progetto

Giuseppe Bellisola
Elisabetta Moratti
Giovannino Silvestri
Claudio Sorio
Professore associato
Marzia Vezzalini
Borsista

Collaboratori esterni

Gianfelice Cinque
Diamond Light Source - UK B22 IR beamlline Principal Beamline Sientist
Paul Dumas
SOLEIL Synchrotron FR - SMIS beamline
Christophe Sandt
SOLEIL Synchrotron FR - SMIS beamline

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