Pubblicazioni

Autori:

Valenti, Maria Teresa; Giannini, S; Donatelli, Luca; Zanatta, Mirko; Bertoldo, Francesco; Sella, S; Vilei, Mt; Ossi, E; Realdi, G; LO CASCIO, Vincenzo; DALLE CARBONARE, Luca Giuseppe

Titolo:

The effect of risedronate on osteogenic lineage is mediated by cyclooxygenase-2 gene upregulation

Anno:

2010

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

Elettronico

Referee:

Sì

Nome rivista:

ARTHRITIS RESEARCH & THERAPY

ISSN Rivista:

1478-6362

N° Volume:

12

Numero o Fascicolo:

4

Intervallo pagine:

R163-R176

Parole chiave:

COX-2; glucocorticoids; histomorphometry; osteoporosis; risedronate

Breve descrizione dei contenuti:

Introduction: The purpose of this study was to evaluate the effects of risedronate (Ris) in the modulation of bone formation in rats with glucocorticoid (GC)-induced osteoporosis by histomorphometric, immunohistochemical and gene expression analyses. Methods: We analyzed structure, turnover and microarchitecture, cyclooxygenase 2 (COX-2) levels and osteocyte apoptosis in 40 female rats divided as follows: 1) vehicle of methylprednisolone (vGC) + vehicle of risedronate (vRis); 2) Ris 5 μg/Kg + vGC; 3) methylprednisolone (GC) 7 mg/Kg + vRis; 4) GC 7 mg/Kg +Ris 5 μg/Kg. In addition, we evaluated cell proliferation and expression of COX-2 and bone alkaline phosphatase (b-ALP) genes in bone marrow cells and MLO-y4 osteocytes treated with Ris alone or in co-treatment with the selective COX-2 inhibitor NS-398 or with dexametasone. Results: Ris reduced apoptosis induced by GC of osteocytes (41% vs 86%, P < 0.0001) and increased COX-2 expression with respect to controls (Immuno-Hystochemical Score (IHS): 8.75 vs 1.00, P < 0.0001). These positive effects of Ris in bone formation were confirmed by in vitro data as the viability and expression of b-ALP gene in bone marrow cells resulted increased in a dose dependent manner. Conclusions: These findings suggest a positive effect of Ris in bone formation and support the hypothesis that the up-regulation of COX-2 could be an additional mechanism of anabolic effect of Ris.

Id prodotto:

58066

Handle IRIS:

11562/346048

depositato il:

29 febbraio 2012

ultima modifica:

15 novembre 2022

Citazione bibliografica:

Valenti, Maria Teresa; Giannini, S; Donatelli, Luca; Zanatta, Mirko; Bertoldo, Francesco; Sella, S; Vilei, Mt; Ossi, E; Realdi, G; LO CASCIO, Vincenzo; DALLE CARBONARE, Luca Giuseppe, The effect of risedronate on osteogenic lineage is mediated by cyclooxygenase-2 gene upregulation «ARTHRITIS RESEARCH & THERAPY» , vol. 12 , n. 4 , 2010 , pp. R163-R176

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