Pubblicazioni

The effect of risedronate on osteogenic lineage is mediated by cyclooxygenase-2 gene upregulation  (2010)

Autori:
Valenti, Maria Teresa; Giannini, S; Donatelli, Luca; Zanatta, Mirko; Bertoldo, Francesco; Sella, S; Vilei, Mt; Ossi, E; Realdi, G; LO CASCIO, Vincenzo; DALLE CARBONARE, Luca Giuseppe
Titolo:
The effect of risedronate on osteogenic lineage is mediated by cyclooxygenase-2 gene upregulation
Anno:
2010
Tipologia prodotto:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Lingua:
Inglese
Formato:
Elettronico
Referee:
Nome rivista:
ARTHRITIS RESEARCH & THERAPY
ISSN Rivista:
1478-6362
N° Volume:
12
Numero o Fascicolo:
4
Intervallo pagine:
R163-R176
Parole chiave:
COX-2; glucocorticoids; histomorphometry; osteoporosis; risedronate
Breve descrizione dei contenuti:
Introduction: The purpose of this study was to evaluate the effects of risedronate (Ris) in the modulation of bone formation in rats with glucocorticoid (GC)-induced osteoporosis by histomorphometric, immunohistochemical and gene expression analyses. Methods: We analyzed structure, turnover and microarchitecture, cyclooxygenase 2 (COX-2) levels and osteocyte apoptosis in 40 female rats divided as follows: 1) vehicle of methylprednisolone (vGC) + vehicle of risedronate (vRis); 2) Ris 5 μg/Kg + vGC; 3) methylprednisolone (GC) 7 mg/Kg + vRis; 4) GC 7 mg/Kg +Ris 5 μg/Kg. In addition, we evaluated cell proliferation and expression of COX-2 and bone alkaline phosphatase (b-ALP) genes in bone marrow cells and MLO-y4 osteocytes treated with Ris alone or in co-treatment with the selective COX-2 inhibitor NS-398 or with dexametasone. Results: Ris reduced apoptosis induced by GC of osteocytes (41% vs 86%, P < 0.0001) and increased COX-2 expression with respect to controls (Immuno-Hystochemical Score (IHS): 8.75 vs 1.00, P < 0.0001). These positive effects of Ris in bone formation were confirmed by in vitro data as the viability and expression of b-ALP gene in bone marrow cells resulted increased in a dose dependent manner. Conclusions: These findings suggest a positive effect of Ris in bone formation and support the hypothesis that the up-regulation of COX-2 could be an additional mechanism of anabolic effect of Ris.
Id prodotto:
58066
Handle IRIS:
11562/346048
depositato il:
29 febbraio 2012
ultima modifica:
15 novembre 2022
Citazione bibliografica:
Valenti, Maria Teresa; Giannini, S; Donatelli, Luca; Zanatta, Mirko; Bertoldo, Francesco; Sella, S; Vilei, Mt; Ossi, E; Realdi, G; LO CASCIO, Vincenzo; DALLE CARBONARE, Luca Giuseppe, The effect of risedronate on osteogenic lineage is mediated by cyclooxygenase-2 gene upregulation «ARTHRITIS RESEARCH & THERAPY» , vol. 12 , n. 42010pp. R163-R176

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo IRIS

Progetti Collegati
Titolo Dipartimento Responsabili
Effetto del risedronato nell'osteoporosi cortisonica su modello animale Dipartimento Medicina Luca Giuseppe Dalle Carbonare
<<indietro

Attività

Strutture

Condividi