Detecting drug effects by Fourier Transform InfraRed microspectroscopy (microFT-IR) on single cells.

Data inizio
2 agosto 2010
Durata (mesi) 
Ingegneria per la medicina di innovazione
Responsabili (o referenti locali)
Bellisola Giuseppe

We will exploit some potential advantages of Fourier Transform InfraRed absorbance microspectroscoy (microFT-IR) to identify biochemical and morphological effects of drugs by SR IR microanalysis on single cells. The goal is to start developing a reference library of validated FTIR spectra via Synchrotron Radiation IR microprobe for the future application of microFT-IR analysis as a drug screening test and for clinical follow-up of patients with leukaemia. We expect to acquire the maximal number of representative spectra with acceptable signal-to-noise ratio (S/N) values for statistics significance in samples. In parallel, complementary biochemical information achieved in samples will be associated with spectral information allowing to cross-validate
reference FT-IR absorbance spectra of functional and structural cellular events induced by drugs in cancer cell models.

Partecipanti al progetto

Elisabetta Moratti
Claudio Sorio
Professore associato

Collaboratori esterni

Gianfelice Cinque
Diamond Light Source - UK B22 IR beamlline Principal Beamline Sientist
Titolo Autori Anno
Tracking InfraRed signatures of drugs in cancer cells by Fourier Transform microspectroscopy Bellisola, Giuseppe; DELLA PERUTA, Marco; Vezzalini, Marzia; Moratti, Elisabetta; Vaccari, L.; Birarda, G.; Piccinini, M.; Cinque, G.; Sorio, Claudio 2010