Pubblicazioni

Autori:

Cigana C.; Mauri P.; Di Silvestre D.; Bergamini G.; Bragonzi A.; Assael B.M.; Sorio C.; Melotti P.

Titolo:

Proteins released from Pseudomonas aeruginosa (Pa) referent and clinical strains under microaerobiosis and their effects on cystic fibrosis airways

Anno:

2008

Tipologia prodotto:

Contributo in atti di convegno

Tipologia ANVUR:

Contributo in Atti di convegno

Lingua:

Inglese

Formato:

A Stampa

Nome rivista:

Journal of Cystic Fibrosis

ISSN Rivista:

1569-1993

N° Volume:

7

Numero o Fascicolo:

suppl 2

Titolo del Convegno:

31st European Cystic Fibrosis Conference,

Luogo:

Prague, Czech Republic

Periodo:

11-14 June 2008

Editore:

Elsevier

Casa editrice:

Elsevier

Intervallo pagine:

S4-S4

Parole chiave:

Pseudomonas Aeruginosa, cystic fibrosis, inflammation

Breve descrizione dei contenuti:

Little is known about changes in protein secretion in Pa laboratory and clinical strains induced by oxygen limitation and their effects on epithelial cells. Here, we demonstrated that conditioned medium from PAO1 and clinical strain AA2 under aerobiosis induced a statistically significant increase of about 2.9 and 4.9 times, respectively of IL-8 and TNF-_ mRNA, in CF airway epithelial cells. This induction was reduced of about 25% when strains were grown under microaerobiosis, suggesting that adaptation to microaerobiosis reduces Pa pathogenicity. Two-dimensional capillary chromatographytandem mass spectrometry (MudPIT) identified 75 polypeptides released by PAO1 and 198 by AA2 in aerobiosis. Differential expression was observed under microaerobiosis when compared to aerobiosis with 6 proteins up-regulated in PAO1 and 7 proteins upregulated in AA2; 4 proteins were downregulated in AA2. 7 proteases were released from AA2 while only 1 from PAO1 with strong down modulation of alkaline metalloprotease in microaerobiosis by AA2 as confirmed by proteolytic activity assay. Identification of virulence factors might be facilitated by this combined approach suggesting molecular targets for pharmacological intervention. Supported by: Italian Cystic Fibrosis Research Foundation FFCgrant17/2006; Comitato Vicenza-Associazione Veneta Lotta contro la Fibrosi Cistica; Azienda Ospedaliera Verona, Italy. Equal contribution: C Cigana and P Mauri.

Id prodotto:

55704

Handle IRIS:

11562/320267

depositato il:

9 aprile 2010

ultima modifica:

2 novembre 2016

Citazione bibliografica:

Cigana C.; Mauri P.; Di Silvestre D.; Bergamini G.; Bragonzi A.; Assael B.M.; Sorio C.; Melotti P., Proteins released from Pseudomonas aeruginosa (Pa) referent and clinical strains under microaerobiosis and their effects on cystic fibrosis airways in «Journal of Cystic Fibrosis» vol. 7 n. suppl 2 Elsevier , Atti di "31st European Cystic Fibrosis Conference," , Prague, Czech Republic , 11-14 June 2008 , 2008 , pp. S4-S4

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo