- Autori:
-
Valenti MT; Dalle Carbonare L; Donatelli L; Bertoldo F; Zanatta M; Lo Cascio V.
- Titolo:
-
Gene expression analysis in osteoblastic differentiation from peripheral blood mesenchymal stem cells.
- Anno:
-
2008
- Tipologia prodotto:
-
Articolo in Rivista
- Tipologia ANVUR:
- Articolo su rivista
- Lingua:
-
Inglese
- Referee:
-
Sì
- Nome rivista:
- Bone
- ISSN Rivista:
- 8756-3282
- N° Volume:
-
43
- Numero o Fascicolo:
-
6
- Intervallo pagine:
-
1084-1092
- Parole chiave:
-
Mesenchymal stem cells; CFU-F; Differentiation
- Breve descrizione dei contenuti:
- MSCs are known to have an extensive proliferative potential and ability to differentiate in various cell types.
Osteoblastic differentiation from mesenchymal progenitor cells is an important step of bone formation,
though the pattern of gene expression during differentiation is not yet well understood. Here, to investigate
the possibility to obtain a model for in vitro bone differentiation using mesenchymal stem cells (hMSCs) from
human subjects non-invasively, we developed a method to obtain hMSCs-like cells from peripheral blood by
a two step method that included an enrichment of mononuclear cells followed by depletion of unwanted
cells. Using these cells, we analyzed the expression of transcription factor genes (runt-related transcription
factor 2 (RUNX2) and osterix (SP7)) and bone related genes (osteopontin (SPP1), osteonectin (SPARC) and
collagen, type I, alpha 1 (COLIA1)) during osteoblastic differentiation. Our results demonstrated that hMSCs
can be obtained from peripheral blood and that they are able to generate CFU-F and to differentiate in
osteoblast and adipocyte; in this study, we also identified a possible gene expression timing during
osteoblastic differentiation that provided a powerful tool to study bone physiology.
- Id prodotto:
-
50441
- Handle IRIS:
-
11562/323871
- depositato il:
-
29 febbraio 2012
- ultima modifica:
-
30 giugno 2020
- Citazione bibliografica:
-
Valenti MT; Dalle Carbonare L; Donatelli L; Bertoldo F; Zanatta M; Lo Cascio V.,
Gene expression analysis in osteoblastic differentiation from peripheral blood mesenchymal stem cells.
«Bone»
, vol.
43
, n.
6
,
2008
,
pp. 1084-1092
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