Pubblicazioni

Autori:

C., Cigana; Nicolis, Elena; Pasetto, Matteo; B. M., Assael; P., Melotti

Titolo:

Effects of azithromycin on the expression of ATP binding cassette transporters in epithelial cells from the airways of cystic fibrosis patients.

Anno:

2007

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Referee:

No

Nome rivista:

Journal of Chemotherapy

ISSN Rivista:

1120-009X

N° Volume:

19

Intervallo pagine:

643-649

Note:

Induction of ATP Binding Cassette (ABC) proteins involved in chloride transport has been proposed as a possible mechanism of the beneficial effects of azithromycin (AZM) in cystic fibrosis (CF) patients. This study focused on the effects of AZM on mRNA and protein expression of Multidrug Resistance-associated Protein 1 (MRP1) and Multidrug Resistance Protein 1 (MDR1) by real-time quantitative PCR, flow cytometry and gene reporter assays in two CF and two isogenic non-CF airway epithelial cell lines. We detected higher levels of MRP1 and lower levels of MDR1 mRNA in CF versus non-CF cells while both proteins were not differentially expressed. After AZM treatment we found modest differences in MRP1 and MDR1 mRNA expression while protein levels were unaffected. The ability of AZM to regulate MRP1 promoter transcriptional activity was excluded by gene reporter assays. Our data do not support the hypothesis of induction of ABC transporters by AZM.

Id prodotto:

48892

Handle IRIS:

11562/327605

depositato il:

27 febbraio 2009

ultima modifica:

23 febbraio 2023

Citazione bibliografica:

C., Cigana; Nicolis, Elena; Pasetto, Matteo; B. M., Assael; P., Melotti, Effects of azithromycin on the expression of ATP binding cassette transporters in epithelial cells from the airways of cystic fibrosis patients. «Journal of Chemotherapy» , vol. 19 , 2007 , pp. 643-649

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