Pubblicazioni

Mouse K-Cl cotransporte KCC1: cloning, mapping, pathological expression and functional regulation.  (1999)

Autori:
Su, W.; Shmukler, B. E.; Chernova, M. N.; Stuart-Tilley, A. K.; De Franceschi, L.; Brugnara, C.; Alper, S. L.
Titolo:
Mouse K-Cl cotransporte KCC1: cloning, mapping, pathological expression and functional regulation.
Anno:
1999
Tipologia prodotto:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Lingua:
Inglese
Formato:
A Stampa
Referee:
Nome rivista:
American Journal of Physiology
ISSN Rivista:
0002-9513
N° Volume:
277
Numero o Fascicolo:
5
Editore:
American Journal of Physiology
Intervallo pagine:
C899-C912
Parole chiave:
Red blood cells; SAD1; SC disease; Sickle cell disease; Thalassemia
Breve descrizione dei contenuti:
Although K-Cl cotransporter (KCC1) mRNA is expressed in many tissues, K-Cl cotransport activity has been measured in few cell types, and detection of endogenous KCC1 polypeptide has not yet been reported. We have cloned the mouse erythroid KCC1 (mKCC1) cDNA and its flanking genomic regions and mapped the mKCC1 gene to chromosome 8. Three anti-peptide antibodies raised against recombinant mKCC1 function as immunoblot and immunoprecipitation reagents. The tissue distributions of mKCC1 mRNA and protein are widespread, and mKCC1 RNA is constitutively expressed during erythroid differentiation of ES cells. KCC1 polypeptide or related antigen is present in erythrocytes of multiple species in which K-Cl cotransport activity has been documented. Erythroid KCC1 polypeptide abundance is elevated in proportion to reticulocyte counts in density-fractionated cells, in bleeding-induced reticulocytosis, in mouse models of sickle cell disease and thalassemia, and in the corresponding human disorders. mKCC1-mediated uptake of (86)Rb into Xenopus oocytes requires extracellular Cl(-), is blocked by the diuretic R(+)-[2-n-butyl-6,7-dichloro-2-cyclopentyl-2, 3-dihydro-1-oxo-1H-indenyl-5-yl-)oxy]acetic acid, and exhibits an erythroid pattern of acute regulation, with activation by hypotonic swelling, N-ethylmaleimide, and staurosporine and inhibition by calyculin and okadaic acid. These reagents and findings will expedite studies of KCC1 structure-function relationships and of the pathobiology of KCC1-mediated K-Cl cotransport.
Pagina Web:
https://doi.org/10.1152/ajpcell.1999.277.5.c899
Id prodotto:
424
Handle IRIS:
11562/424
depositato il:
28 novembre 2007
ultima modifica:
18 ottobre 2021
Citazione bibliografica:
Su, W.; Shmukler, B. E.; Chernova, M. N.; Stuart-Tilley, A. K.; De Franceschi, L.; Brugnara, C.; Alper, S. L., Mouse K-Cl cotransporte KCC1: cloning, mapping, pathological expression and functional regulation. «American Journal of Physiology» , vol. 277 , n. 51999pp. C899-C912

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Mappa proteomica dell'eritrocita: implicazioni cliniche e terapeutiche Dipartimento Medicina Lucia De Franceschi
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